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Educational Support

Classroom Timelines

Experiment: Gel Electrophoresis

FOTODYNE Educational Kits: MolecuLab 105, 106, 107, 108

Suggested Laboratory Timeline:

Day 1: Rehydrate air-dried DNA; cast agarose gels
Day 2: Load and run agarose gels
Day 3: Stain and photograph gels; analyze results

Experiment: Restriction Digestion of DNA

FOTODYNE Educational Kits: MolecuLab 103, 104, 117, 118

Suggested Laboratory Timeline:

Day 1: Rehydrate air-dried DNA; cast agarose gels
Day 2: Restriction digestion of DNA
Day 3: Load and run agarose gels
Day 4: Stain and photograph gels; analyze results

Experiment: Bacterial Transformation

FOTODYNE Educational Kits: MolecuLab 115, 120

Suggested Laboratory Timeline:

Day 1: Streak host bacteria; grow at 37°C overnight
Day 2: Perform heat-shock bacterial transformation; plate
transformed bacteria and grow at 37°C overnight
Day 3: Analyze results; observe phenotypes

Experiment: Polymerase Chain Reaction (PCR)

FOTODYNE Educational Kit: MolecuLab 301

Suggested Laboratory Timeline:

Day 1: Rehydrate air-dried DNA; PCR paper lab exercise OR
DNA purification from food
Day 2: Assemble PCR; run overnight in thermal cycler
Day 3: Load and run gels
Day 4: Stain and photograph gels; analyze results

 

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