Classroom Timelines
Experiment: Gel Electrophoresis
FOTODYNE Educational Kits: MolecuLab 105, 106, 107, 108
Suggested Laboratory Timeline:
Day 1: Rehydrate air-dried DNA; cast agarose gels
Day 2: Load and run agarose gels
Day 3: Stain and photograph gels; analyze results
Experiment: Restriction Digestion of DNA
FOTODYNE Educational Kits: MolecuLab 103, 104, 117, 118
Suggested Laboratory Timeline:
Day 1: Rehydrate air-dried DNA; cast agarose gels
Day 2: Restriction digestion of DNA
Day 3: Load and run agarose gels
Day 4: Stain and photograph gels; analyze results
Experiment: Bacterial Transformation
FOTODYNE Educational Kits: MolecuLab 115, 120
Suggested Laboratory Timeline:
Day 1: Streak host bacteria; grow at 37°C overnight
Day 2: Perform heat-shock bacterial transformation; plate
transformed bacteria and grow at 37°C overnight
Day 3: Analyze results; observe phenotypes
Experiment: Polymerase Chain Reaction (PCR)
FOTODYNE Educational Kit: MolecuLab 301
Suggested Laboratory Timeline:
Day 1: Rehydrate air-dried DNA; PCR paper lab exercise OR
DNA purification from food
Day 2: Assemble PCR; run overnight in thermal cycler
Day 3: Load and run gels
Day 4: Stain and photograph gels; analyze results
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