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Bacterial Strains and Techniques

Bacterial Strains

FOTODYNE Incorporated is pleased to provide the following strains, DNA, probe, primers and media for use with exercises from the laboratory manual Unraveling DNA: Molecular Biology for the Laboratory.

E. coli DH5a Strains

Qty	Description	Exercise	Cat. No.	Price
1 stab	Escherichia coli DH5a	1, 11	E13-1870	$17
1 stab	Escherichia coli DH5a (pUWL500)	6	E13-1871	$17
1 stab	Escherichia coli DH5a (pUWL501)	6	E13-1872	$17
1 stab	Escherichia coli DH5a (pUWL506)	6	E13-1873	$17
1 stab	Escherichia coli DH5a (pUC18)	6	E13-1874	$17
1 stab	Escherichia coli DH5a (pUC19)	6	E13-1875	$17
1 stab	Escherichia coli DH5a (pGEM™-3Zf[+])	1	E13-1877	$17

DNA: Plasmid & Genomic

Qty	Description	Exercise	Cat. No.	Price
1	Plasmid, pUWL500, 50 µg	15,17	E13-1890	$55
1	Plasmid, pUWL501, 50 µg	15, 17	E13-1891	$55
1	Genomic DNA, Vibrio fischeri, 100 µg	8	E13-1899	$60

Bioluminescent Environmental Isolates

Qty	Description	Exercise	Cat. No.	Price
1 stab	Vibrio (photobacterium) fischeri MJ1	1, 5	E13-1886	$17
1 stab	Photobacterium sp. UWL11	1, 23	E13-1878	$17
1 stab	Photobacterium sp. UWL12	23	E13-1879	$17
1 stab	Photobacterium sp. UWL13	23	E13-1880	$17
1 stab	Photobacterium sp. UWL14	23	E13-1881	$17
1 stab	Photobacterium sp. UWL15	23	E13-1882	$17
1 stab	Photobacterium sp. UWL16	23	E13-1883	$17

Probe & Primers

Qty	Description	Exercise	Cat. No.	Price
1	Lux Primer Set (Lux 7 and 8 Primers)	23	E13-1895	$100
1	Lux 7 Primer	2	E13-1889	$55
1	Lux 8 Primer	23	E13-1894	$55
1	Lambda Control Primer Set (Lambda Control Primers 1 and 2)	23	E13-1900	$100
1	Lambda Control Primer 1	23	E13-1896	$55
1	Lambda Control Primer 2	23	E13-1897	$55

Culture Media

Qty	Description	Cat. No.	Price
1	Good Vibrio Medium (GVM) Broth Mix (makes 1L of broth; to make plates add 15 g of agar	E1-1116	$20
1	GVM Agar Plates, pack of 12	E1-1114	$25
1	Photobacterium Broth Mix (makes 1L of broth; to make plates add 15 g of agar	E1-1118	$20
1	Photobacterium Agar Plates, pack of 12	E1-1112	$25

An Innovative Manual of Modern Molecular Biology Techniques

Unraveling DNA: Molecular Biology for the Laboratory, 1st ed.

Michael R. Winfrey,
University of Wisconsin, La Crosse
Marc A. Rott,
University of Wisconsin, La Crosse
Alan Wortman,
Applied Biosystems, Foster City, California

Available through Prentice Hall Publishing

This innovative manual introduces students to all of the basic techniques of modern molecular biology using an integrated series of laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. By organizing the exercises as part of a major cloning project, students get the sense of performing a complete cloning project, rather than just learning a collection of procedures. The manual is divided into discrete units, each demonstrating one or more aspects of the cloning project. Collectively, the entire series of exercises requires approximately three quarters to one full semester to complete with two laboratory periods per week. Smaller portions of the manual are easily adapted to fewer lab periods.

Includes instruction in the basic techniques of modern molecular biology:

DNA isolation and analysis
ligations
restriction mapping
DNA sequencing
transformation

DNA restriction
Southern blotting
hybridization
pulsed field gel electrophoresis

agarose gel electrophoresis
preparation and screening of genomic library

Based on one of nature's most fascinating biological phenomenon: the biological production of light.
Consists of an integrated series of exercises that culminate in the cloning and analysis of the genes coding for bacterial bioluminescence.
All exercises have been thoroughly tested by hundreds of students and over 100 college faculty, including five years of NSF workshops.
Extensive appendices on laboratory safety, care and handling of enzymes, cloning vectors, and suppliers of equipment, materials and reagents.
Instructor's manual includes detailed prep sheets with all materials and equipment needed as well as recipes, sources of materials and reagents, and tips on how to run labs effectively.